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Charge Variants of Trastuzumab using pH Gradient on bioZen 6 µm WCX

Application Detail (App ID: 25061)

Part No:00G-4777-E0
Dimensions:250 x 4.6 mm ID
Elution Type:Gradient
Elution A:CX-1 , pH 5.6
Elution B:CX-2, pH 10.2
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 100 0
2 1 100 0
3 21 0 100
4 23 0 100
7 24 100 0

Flow Rate:1 mL/min
Col. Temp: 25 °C
Detection: LC/UV (DAD, PDA) @ 280.0000000000 nm (nanometers) (0°C)

Analyte Details

Analyte Detail not available

1
Trastuzumab
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