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Imipramine and Amitriptyline Loading on Kinetex 2.6µm PS C18 50x4.6mm using Waters ACQUITY I Class UPLC

Application Detail (App ID: 25512)

Part No:00B-4780-E0
Dimensions:50 x 4.6 mm ID
Elution Type:Gradient
Elution A:Methanol
Elution B:0.1% Formic Acid in ACN
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 75 25
2 2 65 35
3 3 5 95
4 3.1 75 25
5 5 75 25

Flow Rate:1.85 mL/min
Col. Temp: 30 °C
Detection: LC/UV (DAD, PDA) @ 254.0000000000 (30°C)
Note:Imipramine (0.5 mg/mL), with various concentrations of Amitriptyline (0.5, 2.5, 5, and 10%) prepared in water Imipramine (0.5 mg/mL), with various concentrations of Amitriptyline (0.5, 2.5, 5, and 10%) prepared in water

Analyte Details

Analyte Detail not available

1
Imipramine
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2
Amitriptyline
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