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Berberine extracted from Goldenseal pill on Kinetex PS-C18

Application Detail (App ID: 25524)

Part No:00B-4780-E0
Dimensions:50 x 4.6 mm ID
Elution Type:Gradient
Elution A:20mM Potassium dihydrogen phosphate pH 4.6
Elution B:Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 7 35 65
3 8 35 65
4 8.5 90 10
5 10 90 10

Flow Rate:1.5 mL/min
Col. Temp: 23 °C
Detection: LC/UV (DAD, PDA) @ 235.0000000000 nm (nanometers) (0°C)
Note:pill crushed and dissolved in 10mls 50:50 methanol:20 mM phosphate buffer. Sample vortexed and centrifuged. Supernatant transferred to HPLC vial 50:50 methanol:20 mM phosphate buffer

Analyte Details

Analyte Detail not available

1
berberine
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