Applications

Rapid Sample Extraction of Chloroquine, Hydroxychloroquine, and Desethylchloroquine from Serum for LC-MS/MS Analysis

View Tech
Note

Email
Application

Add to App
Locker

Sample Preparation Details

Part Number :CE0-7565

Description :Impact™ Protein Precipitation

Load :150ul of Serum

Evaporate: :Evaporate to dryness

Dispense	450ul of Methanol
Load	150ul of Serum
Vortex	2 minutes
Vacuum	Place an empty collection plate below the Impact protein precipitation plate and pass sample through by applying vacuum, positive pressure, or centrifuge
Add	Dilute filtrate 1:5 with aqueous 0.1 % Formic acid

Application Detail (App ID: 25942)

Column: Kinetex® F5 2.6µm 100 Å, LC Column 50 x 2.1 mm, Ea

Part No:00B-4723-AN

Dimensions:50 x 2.1 mm ID

Elution Type:Gradient

Elution A:0.1 % Formic acid in water

Elution B:Methanol

Gradient Profile:

Step No.	Time (min)	Pct A	Pct B
1	0	98	2
2	2	93	7
3	3	85	15
4	3.2	60	40
5	4.2	5	95
6	6	98	2

Flow Rate:0.5 mL/min

Col. Temp: 20 °C

Detection: LC/MS/MS @ (0°C)

Analyte Details

Analyte Detail not available

Desethylchloroquine

Hydroxychloroquine

Chloroquine