Packing Material | Pore Size (Å) | Effective Surface Area(m2/g) | Effective Carbon Load | pH Stability | Pressure Stability |
F5 | 100 | 200 | 9% | 1.5-8.5 | 1000/600 bar** |
EVO C18 | 100 | 200 | 11% | 1.0-12.0 | 1000/600 bar** |
XB-C18 | 100 | 200 | 10% | 1.5-8.5* | 1000/600 bar** |
C18 | 100 | 200 | 12% | 1.5-8.5* | 1000/600 bar** |
C8 | 100 | 200 | 8% | 1.5-8.5* | 1000/600 bar** |
HILIC | 100 | 200 | 0% | 2.0-7.5 | 1000/600 bar** |
Phenyl-Hexyl | 100 | 200 | 11% | 1.5-8.5* | 1000/600 bar** |
Biphenyl | 100 | 200 | 11% | 1.5-8.5* | 1000/600 bar** |
*pH Stability under gradient conditions. pH stability is 1.5-10 under isocratic conditions.
**2.1 mm ID Kinetex columns are pressure stable up to 1000 bar.
Transform Your HPLC System into a UHPLC or Enhance your UHPLC Methods
Use the Kinetex 2.6 µm Calculator to preview the significant advantages orf increase effiency, resolution, reduced analysis time, and cost savings bu utlizing 2.6µm core-shell technology in place of fully porous particles for HPLC and/or UHPLC methods. For and added benefit, method conditions can be adjusted within the calculator to illustrate the inpact on your separation and lab.
Dramatically Improve Your QC or Existing HPLC Methods
The Kinetex 5 µm Calculator demonstrates how replacing 5 µm fully porous columns with 5 µm core-shell HPLC technology can deliver better chromatography, higher productivity, and cost savings.
Kinetex Care and Use Notes
Learn the best way to maximize performanxce of your Kinetex HPLC/UHPLC column withe the tips below. Alternatively, a PDF of the Kinetex Column Care guide is available in additonal languages.
How Columns are Shipped
Shipping Solvent
- Kinetex® C18, XB-C18, C8, Phenyl-Hexyl and PFP are shipped in Acetonitrile / Water (= 50/50 v/v; exact composition dependent on column dimensions)
- Kinetex HILIC is shipped in Acetonitrile / Water 90:10 (v/v)
Test Certificate
Each Kinetex C18, XB-C18, C8, Phenyl-Hexyl and PFP column is individually tested before shipment. A test certificate showing the separation parameters for the reversed phase test mixture containing uracil, acetophenone, toluene, and naphthalene can be found online.Click Here »
Each Kinetex HILIC column is individually tested before shipment. A test certificate showing the separation parameters for the HILIC test mixture containing toluene, uracil, and cytosine can be found online.Click Here »
Verify to make sure that your Kinetex column is giving perormance similar to the test certificate. Find instructionshere
Operating Parameters
Column Installation
The arrows on the column tag indicate the flow direction. Phenomenex recommends the use of HPLC / UHPLC Sure-Lok™ High Pressure PEEK™ male nut fittings for installation of Kinetex columns on HPLC / UHPLC systems. The convenient one-piece design (AQ0-8503) is pressure rated to 12,000 psi (827 bar), while the 3-piece unit (AQ0-8504 with AQ0-8505), which contains a PEEK nut, a ferrule, and stainless steel gripping ring, will provide leak-free connections up to 19,000 psi (1310 bar). A handy fitting tightening tool (AQ0-8530) is available to facilitate achievement of a leak-free connection.
Installing your Kinetex column on a Waters® AQUITY® UPLC® system? Click here » to see how easy it is!
Mobile Phase Restrictions
- Kinetex C18, XB-C18, C8, Phenyl-Hexyl and PFP columns are stable from pH 1.5 to 8.5 under gradient conditions and can be used with typical reversed phase mobile phase (aqueous methanol, aqueous acetonitrile or appropriate aqueous buffer / methanol or aqueous buffer / acetonitrile mixtures)
- Kinetex HILIC columns are stable from pH 2.0-7.5 and can be used with typical HILIC mobile phase (acetonitrile / aqueous buffer mixtures)
- Kinetex C18, XB-C18, Phenyl-Hexyl and C8 are pH stable from 1.5 – 10 under isocratic conditions.
Use only high purity reagents and high quality chromatography grade solvents to prepare mobile phase. Trace impurities can dramatically degrade column lifetime. Degas and filter all mobile phase prior to use. Ensure sample (and matrix) are completely soluble / miscible with mobile phase. Immiscible solvents or buffer salt precipitation can permanently damage the column.
Avoid:
- Operating below the lower pH limit specified for each Kinetex column as this will hydrolyze the bonded phase
- Operating above the upper pH limit specified for each Kinetex column as this will dissolve the silica
- Immiscible solvents and buffers
- Sudden pressure changes
Operating Backpressure
The maximum operating pressure for Kinetex 2.6 µm columns is 8,700 psi (650 bar)**, and for Kinetex 1.7 µm columns is 15,000 psi (1000 bar). The mobile phase flow rate should be set such that the column backpressure does not exceed this maximum operating pressure. Note that operating at or near the maximum pressure will result in shorter column lifetimes.
**2.1 mm ID Kinetex 2.6 µm columns have a maximum operating pressure 15,000 psi (1000 bar)
Operating Temperatures
Kinetex columns can be used up to a maximum temperature of 60 °C. Reduced mobile phase viscosity (and backpressure), increased mass transfer rates, and enhanced selectivity will be realized above 25 °C. When operating at high pH (> 8), lower operating temperatures are recommended for longer column lifetime. Note that operating at or near the maximum temperature will result in shorter column lifetimes.
Column Cleaning and Regeneration
If an increase in operating backpressure is observed, reverse flush the column (do not try this on other manufacturers’ columns) with reduced flow rates indicated below:
Column ID | Flow Rate |
2.1 mm | 0.1 mL/min |
3.0 mm | 0.3 mL/min |
4.6 mm | 0.5 mL/min |
Reversed Phase (C18, XB-C18, C8, Phenyl-Hexyl, and PFP)
Kinetex reversed phase columns can be cleaned by flushing with 10 to 20 column volumes of:
- 5:95 Acetonitrile / Water (or Methanol / Water) for buffer removal
- 95:5 Acetonitrile / Water (or Methanol / Water)
- THF (Tetrahydrofuran)
- 95:5 Acetonitrile / Water (or Methanol / Water)
- 5:95 Acetonitrile / Water (or Methanol / Water)
- Equilibrate with mobile phase
Column Storage
Reversed Phase (C18, XB-C18, C8, Phenyl-Hexyl, and PFP)
Column storage for a period longer than several days is recommended in ≥ 50 % (v/v) acetonitrile in water or methanol in water. If the mobile phase contained buffer salt, then flush the column with 10 to 20 column volumes of water / acetonitrile or water / methanol to remove the buffer salts before storage. After flushing the column, insert column end plugs securely to prevent evaporation and drying out of the column bed.
HILIC
Column storage for a period longer than several days is recommended in 90 % (v/v) acetonitrile in water. If the mobile phase contained buffer salt, then flush the column with 10 to 20 column volumes of 90:10 acetonitrile / water to remove the buffer salts before storage. After flushing the column, insert column end plugs securely to prevent evaporation and drying out of the column bed.
Get the Most from Your Advanced Kinetex Core-Shell Column!
Achieve better quality data and higher productivity with Kintex Verify! A web tool designed to help you get the most out of the advanced Kinetex core-shell technology.
No Time. No Resources. No Problem.
PhenoLogix Method Development Services is our in-house analytical support laboratory for all your method development and optimization needs. Our customized and confidential approach to supporting your methods will ensure a successful project and your complete satisfaction.
Level 1
Phase Screening
Identify the correct column stationary phase, particle type, and dimensions to give optimum performance.
Level 2
Pre-Validation Service
Perform ICH validation parameters to demonstrate method accuracy, precision, and robustness
Level 3
Onsite Method Transfer
A Phenologix representative will assist with method transfer and demonstrate that the method works in your laboratory.
HPLC System Optimization
Get the most performance out of your HPLC system with some minor system adjustments. See how easy it is to get UHPLC performance on your HPLC system with Kinetex Core-Shell Technology.
Videos
Optimization / Performance Enhancement Kits
This kit comes complete with all the tubing and fittings that is needed to decrease the system dwell volume of your HPLC instrument, instantly improving the observed performance of your Kinetex core-shell column.
Part No. AQ0-8892
Includes the following:
Description | Unit |
PEEKsil™ Tubing 0.100 mm ID x ¹/16 in. OD x 20 cm L, Red | 2/Pk |
PEEKsil™ Tubing 0.100 mm ID x ¹/<sub>16</sub> in. OD x 10 cm L, Red | ea |
Sure-Lok™ High Pressure PEEK™ 1-Pc Male Nut Fitting, 10-32, For ¹/16 in Tubin | 10/Pk |
Sure-Lok™ Male Nut Fitting Tightening Tool, Aluminum | ea |
Column Protection
- Extends Sub-2 µm & core-shell column lifetimes
- For pressures up to 20,000PSI
- Easy to use
Protect Your Investment
With SecurityGuard ULTRA, contaminants and microparticulates are trapped in a guard cartridge, instead of your expensive column. the extremely low dead volumne (<0.3µL) of this unique guard design minimizes sample peak dispersion to maintain column performance without altering your chomratography results.
When contaminants and particulates build up a t the head of your column, system pressure dramatically increases. By simply replacing the SecurityGuard ULTRA cartridge instead of your column you are able to regain normal operating conditions and reclaim original column performance.
See SecurityGuard ULTRA in Action