PREP and DAC Scale Up Calculators | Phenomenex UHPLC, HPLC, SPE and GC

Preparative HPLC
Dynamic Axial Compression (DAC) Column Calculator

DAC Scale Up Calculator

Choose your Bulk Media and Particle Size
Media Density	0

Column	Scout/Prep Column	DAC Column
Length (mm)
Diameter (mm)
Column Volume (empty) (mL)	0	0
Column Bed Volume (empty) (mL)	0	0
Equilibration time (min) (10 C.V.)	0	0
Weight of packing mat (kg)*	0	0
	Scaling Factor	0

Flow rate (mL/min)		0
Sample Amt (g)		0
Injection volume (mL)		0

Notes:

Back pressure stays constant if column length stays constant
Back pressure will increase with decrease in particle size
Efficiency will increase with decrease in particle size
With scale up change in hardware and flow cell may be required

* 0.6 g/mL was used

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*Please note all calculations are approximations only. Several factors influence loading capacity of columns. For example:α , solubility, RT, surface area of material, sample type, instrument, etc.