Region

USP and Ph. Eur.

Allowable adjustments calculator

Ph. Eur. 2.2.46. Allowable Adjustments of Chromatographic Conditions – Isocratic Elution

Component As per Ph. Eur. monograph Adjustable from Adjustable to Limit
Mobile Phase Minor Component ± 30% relative or ± 2% absolute, whichever is the larger; no other component is altered by more than 10% absolute
Mobile Phase pH ±0.2 units
Mobile Phase pH
(If substance to be examined is non-ionizable)
±1 units
Buffer Concentration (mM) ±10%
Column Temperature (°C) ±10°C
Flow Rate (mL/min) ±50% (at same ID)
Column Inner Diameter (mm) ±25%
Column Length (mm) ±70%
Particle Size (µm) - Can be reduced as much as 50%
Stationary Phase

No changes of the identity of the substituent recommended.

Column dimension as per EP Desired Column Dimensions
Column Length (mm)
Column Inner Diameter (mm)
Flow Rate As per Ph. Eur. Monograph (mL/min)
Recommended Flow Rate To Maintain Linear Velocity (mL/min)

Choose Phenomenex columns for your analysis

Selection by :
 Description
 Number
Description according to Pharm. Eur. 10, 4.1.1 Reagents 2020 Number Recommended Phenomenex Column
Choose Ph. Eur column listing

Selected Value: