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Component | As per Ph. Eur. monograph | Adjustable from | Adjustable to | Limit |
---|---|---|---|---|
Mobile Phase Minor Component | ± 30% relative or ± 2% absolute, whichever is the larger; no other component is altered by more than 10% absolute | |||
Mobile Phase pH | ±0.2 units | |||
Mobile Phase pH (If substance to be examined is non-ionizable) |
±1 units | |||
Buffer Concentration (mM) | ±10% | |||
Column Temperature (°C) | ±10°C | |||
Flow Rate (mL/min) | ±50% (at same ID) | |||
Column Inner Diameter (mm) | ±25% | |||
Column Length (mm) | ±70% | |||
Particle Size (µm) | - | Can be reduced as much as 50% | ||
Stationary Phase | No changes of the identity of the substituent recommended. |
Column dimension as per EP | Desired Column Dimensions | |||
Column Length (mm) | ||||
Column Inner Diameter (mm) | ||||
Flow Rate As per Ph. Eur. Monograph (mL/min) | ||||
Recommended Flow Rate To Maintain Linear Velocity (mL/min) |
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Description according to Pharm. Eur. 10, 4.1.1 Reagents 2020 | Number | Recommended Phenomenex Column | ||||
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